pHFX-cas3UD
(Plasmid
#174385)
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PurposePlasmids for gene knockout were constructed based on suicide plasmid pHFX
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHFX
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Vector typearchaeal gene knockout vector for Haloferax mediterranei
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas3UD
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Insert Size (bp)828
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHFX-cas3UD was a gift from Jing Han (Addgene plasmid # 174385 ; http://n2t.net/addgene:174385 ; RRID:Addgene_174385) -
For your References section:
Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux. Lin L, Chen J, Mitra R, Gao Q, Cheng F, Xu T, Zuo Z, Xiang H, Han J. Commun Biol. 2021 Aug 25;4(1):1007. doi: 10.1038/s42003-021-02541-z. 10.1038/s42003-021-02541-z PubMed 34433872