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On-demand biomanufacturing of protective conjugate vaccines.

Stark JC, Jaroentomeechai T, Moeller TD, Hershewe JM, Warfel KF, Moricz BS, Martini AM, Dubner RS, Hsu KJ, Stevenson TC, Jones BD, DeLisa MP, Jewett MC
Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9444. doi: 10.1126/sciadv.abe9444. Print 2021 Feb. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128389pSF-CjPglB-LpxEExpression of C. jejuni PglB and F. tularensis LpxE in E. coli
128390pJL1-MBP-4xDQNATIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites
128391pJL1-PD-4xDQNATIn vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites
128392pJL1-PorA-4xDQNATIn vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites
128393pJL1-TTc-4xDQNATIn vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites
128394pJL1-TTlight-4xDQNATIn vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites
128395pJL1-CRM197-4xDQNATIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites
128397pJL1-EPA-DNNNS-DQNRTIn vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites
128398pTrc99S-ssDsbA-MBP-4xDQNATPeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli
128399pTrc99S-ssDsbA-PD-4xDQNATPeriplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli
128400pTrc99S-ssDsbA-PorA-4xDQNATPeriplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli
128401pTrc99S-ssDsbA-TTc-4xDQNATPeriplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli
128402pTrc99S-ssDsbA-TTlight-4xDQNATPeriplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
128403pTrc99S-ssDsbA-CRM197-4xDQNATPeriplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli
128404pTrc99S-ssDsbA-EPA-DNNNS-DQNRTPeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
132780CLM24 ∆lpxMCLM24 strain with lpxM deletion

Antibodies from Article