Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors.
Rousset F, Cui L, Siouve E, Becavin C, Depardieu F, Bikard D
PLoS Genet. 2018 Nov 7;14(11):e1007749. doi: 10.1371/journal.pgen.1007749. eCollection 2018 Nov.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118727 | FR-E01 | Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome |
| 182740 | pOSIP-KH-RBS2-dCas9 | Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter |