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Addgene

FR-E01
(Bacterial strain #118727)

Full plasmid sequence is not available for this item.

No maps are available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 118727 Bacteria in agar stab 1 $85

Backbone

  • Vector backbone
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli K12 MG1655
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    none

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E. coli K12 MG1655 genotype: F- λ- ilvG- rfb-50 rph-1
Integration at HK022 attB: pOSIP-KH-RBS2-dCas9

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FR-E01 was a gift from David Bikard (Addgene plasmid # 118727)
  • For your References section:

    Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors. Rousset F, Cui L, Siouve E, Becavin C, Depardieu F, Bikard D. PLoS Genet. 2018 Nov 7;14(11):e1007749. doi: 10.1371/journal.pgen.1007749. eCollection 2018 Nov. 10.1371/journal.pgen.1007749 PubMed 30403660