Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.
Mohr MA, Argast P, Pantazis P
Nat Protoc. 2016 Dec;11(12):2419-2431. doi: 10.1038/nprot.2016.134. Epub 2016 Nov 3.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
80609 | p55-H2B-Dendra2 | Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection. |
80904 | pMT-HuC:Dendra2 | Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2 |
82436 | pGEX6P-1-Dendra2 | Dendra2 expression plasmid |