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Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.

Mohr MA, Argast P, Pantazis P
Nat Protoc. 2016 Dec;11(12):2419-2431. doi: 10.1038/nprot.2016.134. Epub 2016 Nov 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
80609p55-H2B-Dendra2Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.
80904pMT-HuC:Dendra2Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2
82436pGEX6P-1-Dendra2Dendra2 expression plasmid

Antibodies from Article