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Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer.

Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
83083TET-pLKO.1 PURO shGDF11 #1Lentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11)
83084TET-pLKO.1 PURO shGDF11 #2Lentiviral shRNA vector for inducible knockdown of human GDF11
83085TET-pLKO.1 PURO shGFPLentiviral shRNA vector for inducible knockdown of GFP (control hairpin)
83086TET-pLKO.1 PURO shID2 #1Lentiviral shRNA vector for inducible knockdown of human ID2
83087TET-pLKO.1 PURO shID2 #2Lentiviral shRNA vector for inducible knockdown of human ID2
83088TET-pLKO.1 PURO shId2 #1Lentiviral shRNA vector for inducible knockdown of mouse Id2
83089TET-pLKO.1 PURO shSMAD4 #1Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)
83090TET-pLKO.1 PURO shId2 #2Lentiviral shRNA vector for inducible knockdown of mouse Id2
83091TET-pLKO.1 PURO shSMAD4 #2Lentiviral shRNA vector for inducible knockdown of human SMAD4
83092TET-pLKO.1 PURO shLucLentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)
83093pEN_TT 3xFLAG LacZGateway entry vector for inducible expression of LacZ with N-terminal 3xFLAG tag
83094pEN_TT 3xFLAG SMAD4Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag
83095pBabe neo TGFBR3-HARetroviral vector for constitutive TGFBR3 expression with C-terminal HA tag
83096pLX302 ID2-V5 puroLentiviral vector for constitutive expression of human ID2 with C-terminal V5 tag
83097pLX302 GDF11-V5 puroLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag
83098pLX304 GDF11-V5 blastLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag
83099pLX304 LacZ-V5 blastLentiviral vector for constitutive expression of LacZ with C-terminal V5 tag
83100pLX304 PCSK5-V5 blastLentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tag
83101pLX304 PCSK5 (T288P)-V5 blastLentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag
83102pLX304 PCSK5 (A270T)-V5 blastLentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag
83103pLX304 PCSK5 (H516P)-V5 blastLentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag
83104pLX304 PCSK5 (R511C)-V5 blastLentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag
83105pSLIK TT 3xFLAG LacZ neoLentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag
83273pSLIK TT 3xFLAG SMAD4 neoLentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag
83274pEN_TT miRc2 3xFLAGEmpty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG
98389pLX302 PCSK5-V5 puroLentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag
98390pEN_TT 3xFLAG ID2Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag
98391pEN_TT 3xFLAG LuciferaseGateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag
98392pSLIK TT 3xFLAG Luciferase neoLentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tag
98393pSLIK TT 3xFLAG ID2 neoLentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag
98394pSLIK TT 3xFLAG ID2 hygroLentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag
98395pSLIK TT 3xFLAG LacZ hygroLentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag
98396pBabe NEO VenusRetroviral vector for constitutive Venus expression
98397pBabe NEO mRFP1Retroviral vector for constitutive mRFP1 expression
98579pLX302 LacZ-V5 puroLentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag
98580pLX304 Luciferase-V5 blastLentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag
98632TET-pLKO.1 PURO shLacZLentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)
98709pLX302 PCSK5 (T288P)-V5 puroLentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Antibodies from Article