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Purpose(Empty Backbone) sgRNA expression in mammalian cells after Gateway cloning
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLL3.7
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Backbone manufacturerAddgene plasmid #11795
- Backbone size (bp) 10054
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Modifications to backboneAddition of hU6 promoter and Gateway cloning cassette
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Vector typeMammalian Expression, Lentiviral
- Promoter hU6
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer U6sponge-F (5'-GGACTATCATATGCTTACCGTAACTTGA-3')
- 3′ sequencing primer StrepPyoCas9-5UTR-F (5'-CGGTGCCACTTTTTCAAGTTG-3') (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-sgRNA-lib 2.0 was a gift from Wensheng Wei (Addgene plasmid # 89638 ; http://n2t.net/addgene:89638 ; RRID:Addgene_89638) -
For your References section:
Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W. Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. 10.1038/nbt.3715 PubMed 27798563