pLenti-sgRNA(lib)-puro
(Plasmid
#119976)
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Purpose(Empty Backbone) Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti-sgRNA-lib 2.0 (Addgene plasmid #89638)
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Backbone manufacturerWensheng Wei Lab
- Backbone size (bp) 9882
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Modifications to backboneReplaced EGFP with puromycin resistance marker
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-sgRNA(lib)-puro was a gift from Wensheng Wei (Addgene plasmid # 119976 ; http://n2t.net/addgene:119976 ; RRID:Addgene_119976) -
For your References section:
Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W. Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283. 10.1038/nbt.4283 PubMed 30395134