pCMV-OptoFGFR1-D387A
(Plasmid
#59778)
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PurposeExpresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by light
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 59778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCitrine-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4733
- Total vector size (bp) 7550
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOptoFGFR1-D387A
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Alt nameLyn-cytoFGFR1-PHR(D387A)-mCit
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SpeciesH. sapiens (human), A. thaliana (mustard weed)
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Insert Size (bp)3617
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GenBank IDNM_023110.2
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Entrez GeneFGFR1 (a.k.a. BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1)
- Promoter CMV
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Tag
/ Fusion Protein
- mCitrine (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-pAR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-OptoFGFR1-D387A was a gift from Won Do Heo (Addgene plasmid # 59778 ; http://n2t.net/addgene:59778 ; RRID:Addgene_59778) -
For your References section:
Spatiotemporal control of fibroblast growth factor receptor signals by blue light. Kim N, Kim JM, Lee M, Kim CY, Chang KY, Heo WD. Chem Biol. 2014 Jul 17;21(7):903-12. doi: 10.1016/j.chembiol.2014.05.013. Epub 2014 Jun 26. 10.1016/j.chembiol.2014.05.013 PubMed 24981772