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PurposeFor optogenetic clustering of FGFR1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111509 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
- Total vector size (bp) 13183
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFGFR1
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SpeciesH. sapiens (human)
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Insert Size (bp)1274
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Entrez GeneFGFR1 (a.k.a. BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1)
- Promoter SFFV
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Tags
/ Fusion Proteins
- Fusion Red (N terminal on backbone)
- Cry2 (N terminal on backbone)
- Myristoylation sequence (MYR) (N terminal on backbone)
- FUS (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FGFR1-optoDroplet was a gift from Jared Toettcher (Addgene plasmid # 111509 ; http://n2t.net/addgene:111509 ; RRID:Addgene_111509) -
For your References section:
Protein Phase Separation Provides Long-Term Memory of Transient Spatial Stimuli. Dine E, Gil AA, Uribe G, Brangwynne CP, Toettcher JE. Cell Syst. 2018 May 24. pii: S2405-4712(18)30190-X. doi: 10.1016/j.cels.2018.05.002. 10.1016/j.cels.2018.05.002 PubMed 29859829