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Addgene

pGEM-HSPA5P
(Plasmid #44150)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44150 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEM
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSPA5 promoter
  • Alt name
    Grp78
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

a fragment containing 429 bp of the human HSPA5 promoter
(-282 to +147) relative to the transcriptional start site) was amplified from human genomic DNA by PCR and cloned into pGEM-T Easy Vector (Promega) to generate pGEM-HSPA5P

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-HSPA5P was a gift from Joan Conaway (Addgene plasmid # 44150 ; http://n2t.net/addgene:44150 ; RRID:Addgene_44150)
  • For your References section:

    Endoplasmic reticulum stress-responsive transcription factor ATF6alpha directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes. Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, Conaway RC. J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 10.1074/jbc.M112.369504. Epub 2012 May 10. 10.1074/jbc.M112.369504 PubMed 22577136