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PurposeMammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBiP
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SpeciesH. sapiens (human)
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GenBank IDNP_005338.1
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Entrez GeneHSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
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Tags
/ Fusion Proteins
- Myc (C terminal on insert)
- KDEL (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV BiP-Myc-KDEL-wt was a gift from Ron Prywes (Addgene plasmid # 27164 ; http://n2t.net/addgene:27164 ; RRID:Addgene_27164) -
For your References section:
ER stress regulation of ATF6 localization by dissociation of BiP/GRP78 binding and unmasking of Golgi localization signals. Shen J, Chen X, Hendershot L, Prywes R. Dev Cell. 2002 Jul . 3(1):99-111. 10.1016/S1534-5807(02)00203-4 PubMed 12110171