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PurposeExpresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Modifications to backboneThe luc2=tdT fusion gene was created by PCR amplification of the Luc2 gene (pGL4.10, Promega, Madison, WI) and cloned by restriction digestion upstream of the tdTomato gene in the vector pRSET-B-tdTomato (kindly provided by Roger Tsien, UC San Diego). The luc2=tdT fusion gene was excised with EcoRI and inserted into the EcoRI site in the pcDNA3.1(+) multiple cloning site.
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameFirefly Luciferase
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Alt nameluc2
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SpeciesPhotonis pyralis (firefly)
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Insert Size (bp)3129
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GenBank IDAY738222
- Promoter CMV-IE
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Tag
/ Fusion Protein
- tdTomato red fluorescent protein (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nametandem Tomato Red Fluorescent Protein
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SpeciesDiscosoma
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GenBank IDAY678269
- Promoter n/a
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer pcDNA3.1/BGH reverse primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis luc2=tdT (luciferase-tandem Tomato Red Fluorescent Protein) fusion gene was cloned by the submitter, Dr. Michael H. Bachmann, Dept. of Pediatrics, Stanford University, Stanford, CA 94305-5427.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)/Luc2=tdT was a gift from Christopher Contag (Addgene plasmid # 32904 ; http://n2t.net/addgene:32904 ; RRID:Addgene_32904) -
For your References section:
Longitudinal, noninvasive imaging of T-cell effector function and proliferation in living subjects. Patel MR, Chang YF, Chen IY, Bachmann MH, Yan X, Contag CH, Gambhir SS. Cancer Res. 2010 Dec 15;70(24):10141-9. 10.1158/0008-5472.CAN-10-1843 PubMed 21159636