MCV-Rep-
(Plasmid
#32058)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32058 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJ241
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Backbone manufacturerDNA2.0
- Backbone size w/o insert (bp) 2755
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCV-Rep- genome
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SpeciesMerkel cell polyomavirus
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Insert Size (bp)5387
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MutationSingle cytosine to adenine (C/A) mutation at 2,073 (nt) of full construct, which deletes viral replication function
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GenBank IDJF813003
- Promoter none from vector. Insert contains native promoter from viral genome encompassed in the viral non-coding control region.
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrFI (not destroyed)
- 3′ cloning site BsrFI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCV-Rep- was a gift from Patrick Moore (Addgene plasmid # 32058 ; http://n2t.net/addgene:32058 ; RRID:Addgene_32058) -
For your References section:
Cellular and viral factors regulating merkel cell polyomavirus replication. Feng H, Kwun HJ, Liu X, Gjoerup O, Stolz DB, Chang Y, Moore PS. PLoS One. 2011;6(7):e22468. Epub 2011 Jul 22. 10.1371/journal.pone.0022468 PubMed 21799863