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Addgene

pCDNA3 Flag p38 delta
(Plasmid #20785)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20785 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pDCNA3 Flag p38 delta
  • Alt name
    Mapk13
  • Alt name
    Sapk4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1998
  • GenBank ID
    NP_036080
  • Entrez Gene
    Mapk13 (a.k.a. SAPK4, Serk4)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original wild-type p38 delta insert provided by Ulevitch, R.J. and Han,J, "Characterization of the Structure and function of the fourth member of p38 group mitogen-activated protein kinase pathway",(J.BiolChem.272(6),3296-3301 (1997).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 Flag p38 delta was a gift from Roger Davis (Addgene plasmid # 20785 ; http://n2t.net/addgene:20785 ; RRID:Addgene_20785)
  • For your References section:

    Molecular determinants that mediate selective activation of p38 MAP kinase isoforms. Enslen H, Brancho DM, Davis RJ. EMBO J. 2000 Mar 15. 19(6):1301-11. 10.1093/emboj/19.6.1301 PubMed 10716930