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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepDCNA3 Flag p38 delta
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Alt nameMapk13
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Alt nameSapk4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1998
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GenBank IDNP_036080
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Entrez GeneMapk13 (a.k.a. SAPK4, Serk4)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byoriginal wild-type p38 delta insert provided by Ulevitch, R.J. and Han,J, "Characterization of the Structure and function of the fourth member of p38 group mitogen-activated protein kinase pathway",(J.BiolChem.272(6),3296-3301 (1997).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3 Flag p38 delta was a gift from Roger Davis (Addgene plasmid # 20785 ; http://n2t.net/addgene:20785 ; RRID:Addgene_20785) -
For your References section:
Molecular determinants that mediate selective activation of p38 MAP kinase isoforms. Enslen H, Brancho DM, Davis RJ. EMBO J. 2000 Mar 15. 19(6):1301-11. 10.1093/emboj/19.6.1301 PubMed 10716930