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Addgene

pCDNA3 Flag p38 gamma
(Plasmid #20353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20353 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pDCNA3 Flag p38 gamma
  • Alt name
    MapK12
  • Alt name
    SAPK3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1175
  • GenBank ID
    NM_021746
  • Entrez Gene
    Mapk12 (a.k.a. Sapk3)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xho 1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 Flag p38 gamma was a gift from Roger Davis (Addgene plasmid # 20353 ; http://n2t.net/addgene:20353 ; RRID:Addgene_20353)

  • For your References section:

    Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6. Enslen H, Raingeaud J, Davis RJ. J Biol Chem. 1998 Jan 16. 273(3):1741-8. 10.1074/jbc.273.3.1741 PubMed 9430721
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