pMC0x_gRNA_Pos5b2_Ptet
(Plasmid
#179342)
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PurposegRNA Position Vector 5b2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepMC_V*_01_LVL0* Entry sfGFP (Marburg Collection)
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePtet gRNA with sfGFP dropout
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SpeciesA. victoria
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.08.02.454823v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMC0x_gRNA_Pos5b2_Ptet was a gift from Anke Becker (Addgene plasmid # 179342 ; http://n2t.net/addgene:179342 ; RRID:Addgene_179342) -
For your References section:
NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens. Stukenberg D, Hoff J, Faber A, Becker A. Commun Biol. 2022 Mar 25;5(1):265. doi: 10.1038/s42003-022-03150-0. 10.1038/s42003-022-03150-0 PubMed 35338236