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Addgene

pST_140_LVL2 cam
(Plasmid #179334)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom built using Marburg Collection
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
  • Species
    E. coli, V. cholerae, S. pyogenes, Bacteriophage, A. victoria
  • Mutation
    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST_140_LVL2 cam was a gift from Anke Becker (Addgene plasmid # 179334 ; http://n2t.net/addgene:179334 ; RRID:Addgene_179334)

  • For your References section:

    NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens. Stukenberg D, Hoff J, Faber A, Becker A. Commun Biol. 2022 Mar 25;5(1):265. doi: 10.1038/s42003-022-03150-0. 10.1038/s42003-022-03150-0 PubMed 35338236
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