pET30a-SARS-CoV2-ORF3b (S23Q)
(Plasmid
#165088)
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Purposeprotein expression in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 165088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET-30a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
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SpeciesSARS-CoV2
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MutationStop codon to glutamine at 23rd amino acid
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET30a-SARS-CoV2-ORF3b (S23Q) was a gift from Rick Tarleton (Addgene plasmid # 165088 ; http://n2t.net/addgene:165088 ; RRID:Addgene_165088) -
For your References section:
A flexible, pan-species, multi-antigen platform for the detection and monitoring of SARS-CoV-2-specific antibody responses. Shen H, Forgacs D, Chapla D, Moremen KW, Wells L, Hamer SA, Tompkins SM, Ross TM, Rouphael N, Edupuganti S, Collins MH, Tarleton RL. medRxiv [Preprint]. 2021 Jan 27:2021.01.20.21249279. doi: 10.1101/2021.01.20.21249279. 10.1101/2021.01.20.21249279 PubMed 33532799