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Addgene

pcDNA3.1-ACE2-GFP
(Plasmid #154962)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154962 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone size w/o insert (bp) 5428
  • Modifications to backbone
    Addgene 1786: replaced C9 c-terminal tag for EGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACE2-GFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3141
  • GenBank ID
    NM_021804
  • Entrez Gene
    ACE2 (a.k.a. ACEH)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpni (not destroyed)
  • 3′ cloning site AflII (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene 1786
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-ACE2-GFP was a gift from Utpal Pajvani (Addgene plasmid # 154962 ; http://n2t.net/addgene:154962 ; RRID:Addgene_154962)
  • For your References section:

    Angiotensin converting enzyme 2 is a novel target of the gamma-secretase complex. Bartolome A, Liang J, Wang P, Ho DD, Pajvani UB. Sci Rep. 2021 May 7;11(1):9803. doi: 10.1038/s41598-021-89379-x. 10.1038/s41598-021-89379-x PubMed 33963249