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PurposepcDNA3.1-ACE2-GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154962 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(-)
- Backbone size w/o insert (bp) 5428
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Modifications to backboneAddgene 1786: replaced C9 c-terminal tag for EGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACE2-GFP
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SpeciesH. sapiens (human)
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Insert Size (bp)3141
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GenBank IDNM_021804
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Entrez GeneACE2 (a.k.a. ACEH)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpni (not destroyed)
- 3′ cloning site AflII (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene 1786
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-ACE2-GFP was a gift from Utpal Pajvani (Addgene plasmid # 154962 ; http://n2t.net/addgene:154962 ; RRID:Addgene_154962) -
For your References section:
Angiotensin converting enzyme 2 is a novel target of the gamma-secretase complex. Bartolome A, Liang J, Wang P, Ho DD, Pajvani UB. Sci Rep. 2021 May 7;11(1):9803. doi: 10.1038/s41598-021-89379-x. 10.1038/s41598-021-89379-x PubMed 33963249