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Addgene

pET-21a(+)-IS200
(Plasmid #12604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    IS200 transposase
  • Alt name
    SSO1474
  • Species
    Sulfolobus solfataricus
  • Insert Size (bp)
    399
  • GenBank ID
    AAK41701
  • Entrez Gene
    SSO1474

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-21a(+)-IS200 was a gift from SeWon Suh (Addgene plasmid # 12604 ; http://n2t.net/addgene:12604 ; RRID:Addgene_12604)

  • For your References section:

    Crystal structure of a metal ion-bound IS200 transposase. Lee HH, Yoon JY, Kim HS, Kang JY, Kim KH, Kim DJ, Ha JY, Mikami B, Yoon HJ, Suh SW. J Biol Chem. 2006 Feb 17. 281(7):4261-6. 10.1074/jbc.M511567200 PubMed 16340015
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