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Addgene

pJTX-GL3
(Plasmid #11981)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11981 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-jun promoter
  • Alt name
    c-jun
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    375
  • Entrez Gene
    Jun (a.k.a. AP-1, Junc, c-jun)
  • Tag / Fusion Protein
    • Luc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids pJC6GL3, pJSXGL3, pJTXGL3, and pJSTXGL3 contain positions -225 to +150 of the murine c-jun promoter. pJSXGL3, pJTXGL3, and pJSTXGL3 contain mutations at the MEF2 site, ATF (AP1-like) site, and both sites, respectively.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJTX-GL3 was a gift from Ron Prywes (Addgene plasmid # 11981 ; http://n2t.net/addgene:11981 ; RRID:Addgene_11981)
  • For your References section:

    Epidermal growth factor induction of the c-jun promoter by a Rac pathway. Clarke N, Arenzana N, Hai T, Minden A, Prywes R. Mol Cell Biol. 1998 Feb . 18(2):1065-73. PubMed 9448004