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Addgene

phage UbiC NLS HA stdMCP stdHalo
(Plasmid #104999)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104999 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHAGE-UbiC
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    stdMCP-stdHalo
  • Species
    Synthetic
  • Insert Size (bp)
    2628
  • Promoter UbiC
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • HA tag (N terminal on insert)
    • Two synonymous copies of the MS2 coat protein (N terminal on insert)
    • Two synonymous copies of the Halo-tag

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phage UbiC NLS HA stdMCP stdHalo was a gift from Jeffrey Chao (Addgene plasmid # 104999 ; http://n2t.net/addgene:104999 ; RRID:Addgene_104999)
  • For your References section:

    Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008. 10.1016/j.celrep.2017.12.008 PubMed 29281824