-
PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 104999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHAGE-UbiC
-
Vector typeMammalian Expression, Bacterial Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namestdMCP-stdHalo
-
SpeciesSynthetic
-
Insert Size (bp)2628
- Promoter UbiC
-
Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- HA tag (N terminal on insert)
- Two synonymous copies of the MS2 coat protein (N terminal on insert)
- Two synonymous copies of the Halo-tag
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GTGTGGCACAGCTAGTTCC (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
phage UbiC NLS HA stdMCP stdHalo was a gift from Jeffrey Chao (Addgene plasmid # 104999 ; http://n2t.net/addgene:104999 ; RRID:Addgene_104999) -
For your References section:
Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008. 10.1016/j.celrep.2017.12.008 PubMed 29281824