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PurposepSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 84561 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUbC lentivirus expression vector (2nd generation)
- Backbone size w/o insert (bp) 6255
- Total vector size (bp) 11219
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
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Alt nameSINAPS
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SpeciesM. musculus (mouse), A. thaliana (mustard weed), Synthetic
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Insert Size (bp)4964
- Promoter UbC
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Tag
/ Fusion Protein
- Flag, Suntag, oxBFP, AID (auxin-inducible degron)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site ClaI--but may not be unique due to multiple inserts (not destroyed)
- 5′ sequencing primer cgc cga tga tta tat aag ga (UbC)
- 3′ sequencing primer cat aaa gag aca gca acc ag (Wpre) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre was a gift from Robert Singer (Addgene plasmid # 84561 ; http://n2t.net/addgene:84561 ; RRID:Addgene_84561) -
For your References section:
Translation dynamics of single mRNAs in live cells and neurons. Wu B, Eliscovich C, Yoon YJ, Singer RH. Science. 2016 Jun 17;352(6292):1430-5. doi: 10.1126/science.aaf1084. Epub 2016 May 5. 10.1126/science.aaf1084 PubMed 27313041