Adenovirus Plasmids

Adenoviruses are a class of medium-sized, non-enveloped, double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target a broad range of dividing and non-dividing cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses, adenoviruses do not integrate into the host genome. Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune response induced by the viral capsid.

Adenoviruses can be produced by several methods, the most common of which involves homologous recombination of adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule can then be transfected into mammalian packaging cell lines to generate adenovirus particles.

Read our Adenoviral Guide for more information about:

• Adenoviral components
• Adenoviral plasmid construction
• Frequently asked questions about adenovirus
• Glossary of commonly used adenovirus terms

Adenoviral Strains

Bacterial strains for generating adenovirus.

	ID	Strain	Description	PI
	16398	BJ5183	Strain for recombination between shuttle plasmid and adenoviral plasmid	Vogelstein
	16399	AdEasier-1	BJ5183 derivative that contains AdEasy-1™ plasmid	Vogelstein

Adenoviral Plasmids

Common shuttle and adenoviral plasmids. Want an adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have inserts.

	ID	Plasmid	Type	Description	PI
	16400	pAdEasy-1	Adenoviral	For recombining shuttle plasmids	Vogelstein
	16401	pAdEasy-2	Adenoviral	For recombining shuttle plasmids; for use with large inserts	Vogelstein
	179201	pAd5-B6 ∆E3-GFP	Adenoviral	A block for the AdenoBuilder genome assembly system. Block 6 with deletion in E3 and insertion of GFP expression cassette.	Bunz
	179202	pAd5-B6/7 ∆E3-GFP	Adenoviral	A block for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5-B6 ∆E3-GFP and pAd5-B7.	Bunz
	179203	pAd5-B1-deltaE1-GFP	Adenoviral	A block for the AdenoBuilder genome assembly system. Block 1 with deletion in E1 and insertion of GFP expression cassette.	Bunz
	16402	pShuttle	Shuttle	For production of viruses containing transgene under a chosen promoter	Vogelstein
	16403	pShuttle-CMV	Shuttle	For production of viruses containing transgene under CMV promoter	Vogelstein
	16404	pAdTrack	Shuttle	For production of GFP-trackable viruses containing transgene under a chosen promoter	Vogelstein
	16405	pAdTrack-CMV	Shuttle	For production of GFP-trackable viruses containing transgene under CMV promoter	Vogelstein
	16407	pAdEasy 2-GFP beta-gal	Shuttle	Test plasmid that contains β-gal and GFP; contains ∼10Kb genomic DNA stuffer	Vogelstein
	18703	pEZYshuttle	Shuttle	Modified pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids	Zhang
	50957	RedTrackCMV	Shuttle	For production of mRFP-trackable viruses containing transgene under CMV	Bamburg
	50958	ShuttleNSE	Shuttle	For production of viruses containing transgene under Neuron-specific enolase (NSE) promoter	Bamburg
	50959	ShuttleMCP	Shuttle	For production of viruses containing transgene under mouse cofilin promoter (MCP)	Bamburg
	62621	pShuttle-CMV-F2A-T2A-Venus	Shuttle	For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without Venus	Wang

Adenoviral Kits

Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.

ID	Kit	Description	PI
1000000176	AdenoBuilder toolkit	Plasmids contain modular inserts for seamless assembly of multiple mutations, alterations, or transgene insertions into single full-length adenovirus genomes	Bunz