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AAV Packaged on Request

Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely handled at the lowest biosafety protection levels.

We offer 955 in-stock viral vector preps, delivering high quality and ready availability at affordable prices. While our repository serves the needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities. To meet that need, our Packaged on Request service lets scientists choose from over 3,000 transfer plasmids to be packaged in their choice of five serotypes, resulting in over 15,000 possible choices!

By using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and titer viral vectors.

Overview

Our new Packaged on Request service is recommended for research-grade use. Many chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this service.

Select any eligible plasmid in the Addgene repository in your choice of:

  • serotype (AAV1, AAV2, AAV5, AAV8, or AAVrg) and
  • volume (1 mL, 2.5 mL, or 5 mL).

Currently, we can only package plasmids in our collection in the serotypes listed here.

Details

Scale and Titer

Packaged on Request can be ordered in three sizes. For all viral vector preparations, we set both minimum standards and typical titers, though actual yields may vary by construct.

Size Quantity Total Volume Minimum Titer Typical Titer
Small 10 × 100 µL aliquots 1 mL 4 × 1012 GC/mL* 1 × 1013 GC/mL*
Medium 25 × 100 µL aliquots 2.5 mL
Large 50 × 100 µL aliquots 5 mL

*AAV2 yields are generally lower, with a minimum titer of 2 × 1012 GC/mL and a typical titer of 7 × 1012 GC/mL.

For more details, see our Scope of Service.

All-in-One Pricing

The price for our Packaged on Request preps is all-inclusive of several important steps:

  • MTA facilitation for the transfer plasmid, including legal approval and an implementation letter
  • DNA amplification
  • Viral vector production
  • Density centrifugation purification
  • Addgene’s comprehensive quality control process:
    • Titering
    • Purity assessment
    • Sterility testing
    • Viral genome sequencing (VGS)

Find detailed pricing for your chosen plasmid on its material page.

Request and Ordering Process

AAV production will take approximately 6–8 weeks. Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that the actual time from request to delivery could be up to 10 weeks.

This breaks down as follows:

Request
1–2 days
Look for the banner on an eligible plasmid page and place your request. We will send you an email within 1-2 days with a response. Most requests will be approved within two business days!
Ordering
At your convenience
After approval, the next step is yours: you must place your order to begin production. You can do this whenever you are ready.
MTA Approval
1+ business days, varies by requesting organization
Viral vector preps remain subject to the terms and conditions of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the providing scientist through an MTA. This process typically takes 1–4 days, depending on your institution.
AAV Production
6–8 weeks
Quality is our priority. During this time, we amplify DNA, produce the prep, and perform comprehensive quality control processes to make sure it is ready for your experiments.
Shipping
2–7 days
We will email you with tracking information as soon as your order ships. Shipping time will depend on your location.

Share Your Results

Once you start getting results from your AAV experiments, remember to share your protocols and results through Addgene’s Data Hub. By contributing your AAV vector experience, from methods to troubleshooting insights, you make research more reproducible for scientists everywhere and help build a globally connected scientific community. The Data Hub captures valuable knowledge that often goes unshared, bridging the gap between published methods and real-world troubleshooting.

Eligible Plasmids

The following plasmids are eligible for this service. Submit a request and we’ll let you know whether the item can be packaged.

Addgene does not perform functional or in vivo testing. We recommend that you consult the original paper of your chosen AAV plasmid for more information on its performance.

ID Plasmid Gene/Insert Promoter PI

Additional Resources

Viral Vector Production
Learn about how we produce high-quality viral vector preps at Addgene, including purification, titration, and quality control measures. We have distributed preps around the globe and shared our well-developed and trusted protocols and peer-reviewed techniques (Link opens in a new window) for producing high-quality research-grade viral vector preps.
Data Hub
Access Addgene’s Data Hub for protocols and results shared by other scientists using AAV vectors. Our community-driven Data Hub connects researchers with troubleshooting and results that complement published data, capturing the insights that often go unshared.
Help Center
Check out Addgene’s Help Center, a searchable database of information to help you every step of the way, including ordering, handling materials, using them in the lab, and even managing legal permissions and distribution.
Troubleshooting
If your answer isn’t in the Help Center or you need more assistance with troubleshooting, we want to hear from you! Email us at [email protected].