Browse Lentivirus
Addgene's lentiviruses are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the inventory below.
Lentiviruses are distributed as purified viral particles in solution that are suitable for in vivo use. Volume and minimum titers are listed on each item's material page. Actual titers are reported with each shipment.
For more details about our viral vector packaging service, visit our Viral Vector Packaging Service page.
Category
Cas9 Function
Pooled CRISPR Libraries for Screening
Use pooled CRISPR libraries to screen for genes involved in specific biological processes. For more information, see our Guide to Plasmid Pooled Libraries.
| ID | Name | Description | Genes/Insert | Selection | PI |
|---|---|---|---|---|---|
| Human knockout pooled libraries | |||||
| 73178 | Human sgRNA library Brunello in lentiGuide-Puro | Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. | 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls | Puromycin | Doench, Root |
| 73179 | Human gRNA pooled library in lentiCRISPRv2 | Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. | SpCas9 and 76,441 unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls | Puromycin | Doench, Root |
| Human activation pooled libraries | |||||
| 92379 | Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A | Human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) targeting 18,885 genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. | 56,762 unique sgRNAs targeting 18,885 human genes | Puromycin | Doench, Root |
| Human inactivation pooled libraries | |||||
| 92385 | Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A | Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,901 genes and containing 57,050 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. | 57,050 unique sgRNAs targeting 18,901 human genes along with 500 non-targeting controls | Puromycin | Doench, Root |
| 92386 | Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set B | Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,899 genes and containing 57,011 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. | 57,011 unique sgRNAs targeting 18,899 human genes along with 500 non-targeting controls | Puromycin | Doench, Root |
| Mouse knockout pooled libraries | |||||
| 73633 | Mouse sgRNA library Brie in lentiGuide-Puro | Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls. This backbone does not contain SpCas9. | 78,637 unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls | Puromycin | Doench, Root |
Cas9 Lentivirus
Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page.
Cas9 for Editing Genomic Sequences
To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks (DSBs or cuts) or single-stranded breaks (nicks).
| ID | Name | Insert | Function | Antibiotic Resistance | Activity | PI |
|---|---|---|---|---|---|---|
| 52962 | lentiCas9-Blast | Sp Cas9 | Cut | Blasticidin | Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. | Zhang |
Cas9 and Accessories for Activating Gene Expression
Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator.
| ID | Name | Insert | Antibiotic Resistance | Activity | PI |
|---|---|---|---|---|---|
| 61422 | dCAS9-VP64_GFP | dCAS9 (D10A, H840A) | none | Expresses dCAS9-VP64 activator with 2A GFP | Zhang |
| 61425 | lenti dCAS-VP64_Blast | dCAS9 (D10A, N863A) | Blasticidin | 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) | Zhang |
| 61426 | lenti MS2-P65-HSF1_Hygro | MS2-P65-HSF1 | Hygromycin | lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) | Zhang |
Pooled Barcoding Libraries
| ID | Name | Description | PI |
|---|---|---|---|
| 115643 | Morris CellTag Barcode Library Version 1 | Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization via direct fluorescence. | Morris |
| 115644 | Morris CellTag Barcode Library Version 2 | Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding library. Version 2 of the Celltag library contains 4934 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization via direct fluorescence. | Morris |
| 115645 | Morris CellTag Barcode Library Version 3 | Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding library. Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization via direct fluorescence. | Morris |
Control Lentivirus
Browse our list of available viruses based on plasmids in our repository that express fluorescent proteins.
| ID | Name | Insert | Antibiotic Resistance | Purpose | PI |
|---|---|---|---|---|---|
| 17446 | pLenti CMV GFP Hygro (656-4) | GFP | Hygromycin | 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro | Campeau |
COVID-19 Research Related Lentivirus
Browse our list of available viruses based on plasmids in our repository that are related to COVID-19 research.
| ID | Name | Description | PI |
|---|---|---|---|
| 141391 | pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro | Expression of SARS-CoV-2 nucleocapsid (N) protein | Krogan |
| 141386 | pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro | Expression of SARS-CoV-2 membrane (M) protein | Krogan |
| 141385 | pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro | Expression of SARS-CoV-2 envelope (E) protein | Krogan |
| 141383 | pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro | Expression of SARS-CoV-2 orf3a protein | Krogan |
| 141347 | pBOB-CAG-SARS-CoV2-Spike-HA | Expression of SARS-CoV-2 spike (S) protein | Pao |
| 154985 | pWPI-IRES-Puro-Ak-ACE2 | Expression of human ACE2 | Best |
| 154982 | pWPI-IRES-Bla-Ak-TMPRSS2 | Expression of human TMPRSS2 | Best |
| 153011 | ACE2 g1 + Cas9 | Expression of Cas9 + ACE2 gRNA | Sheltzer |
| 153015 | TMPRSS2 g1 + Cas9 | Expression of Cas9 + TMPRSS2 gRNA | Sheltzer |
Don’t see what you are looking for? Please let us know what you would like to see available in viral format by filling out our Viral Service Suggestion Form (Link opens in a new window) .
Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.