Viral Vector Packaging Service: Caltech Systemic Capsids
As part of our Viral Vector Packaging Service, Addgene is distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in a new window). As part of our standard viral production, these viral vectors undergo quality control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation.
Systemic Delivery AAV Capsid Choice
Need help deciding which capsid to choose? The following decision tree was developed at the CLOVER Center at Caltech and can help guide your decision.
For more in depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids. To review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository of published capsid use (Link opens in a new window).
PHP.eB
These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005).
Note on PHP.eB tropism and specific mouse lines: Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (Huang et al., 2019 (Link opens in a new window)) to cross the blood brain barrier. For information on whether the PHP.eB serotype will work in your mouse line, see Supplementary Table 3 (Link opens in a new window).
Browse Available PHP.eB AAV
ID | Name | Promoter | Description | Category | PI |
---|---|---|---|---|---|
Controls | |||||
28306 | pAAV-FLEX-tdTomato | CAG | tdTomato, Cre-dependent | Control | Boyden |
37825 | pAAV-CAG-GFP | CAG | GFP | Control | Boyden |
50469 | pAAV-CaMKIIa-EGFP | CamKIIa | EGFP | Control | Roth |
59462 | pAAV-CAG-tdTomato | CAG | tdTomato | Control | Boyden |
83900 | pAAV-mDlx-GFP-Fishell-1 | Dlx | GFP | Control | Fishell |
99130 | pAAV-mDlx-NLS-mRuby2 | Dlx | mRuby2 | Control | Gradinaru |
104055 | pAAV-CAG-eYFP | CAG | EYFP | Control | Gradinaru |
104061 | CAG-NLS-GFP | CAG | NLS-GFP | Control | Gradinaru |
105530 | pAAV.CMV.PI.EGFP.WPRE.bGH | CMV | EGFP | Control | Wilson |
105547 | pENN.AAV.EF1a.eGFP.WPRE.rBG | EF1a | EGFP | Control | Wilson |
114469 | pAAV-CaMKIIa-mCherry | CamKIIa | mCherry | Control | Deisseroth |
114470 | pAAV-Ef1a-mCherry | EF1a | mCherry | Control | Deisseroth |
117382 | hSyn1-eYFP | Syn | EYFP | Control | Gradinaru |
135630 | pAAV-S5E2-dTom-nlsdTom | E2 regulatory element | dTomato | Control | Dimidschstein |
135631 | pAAV-S5E2-GFP-fGFP | E2 regulatory element | GFP, membrane-targeted GFP | Control | Dimidschstein |
192552 | pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA | CAG | tdTomato | Control | Feng |
163505 | CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA | minBetaGlobin | SYFP2 | Control | Ting |
163509 | CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA | hSyn1 | H2B-SYFP2 | Control | Ting |
191706 | AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) | minBG | SYFP2 | Control | AIBS, Ting |
191720 | AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) | minBG | SYFP2 | Control | AIBS, Ting |
191728 | AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) | minBG | SYFP2 | Control | AIBS, Ting |
199773 | AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) | minBG | SYFP2 | Control | AIBS, Ting |
199774 | AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905) | minBG | SYFP2 | Control | AIBS, Ting |
Chemogenetics | |||||
44361 | pAAV-hSyn-DIO-hM3D(Gq)-mCherry | Syn | Activator, Cre-dependent | DREADD | Roth |
44362 | pAAV-hSyn-DIO-hM4D(Gi)-mCherry | Syn | Inhibitor, Cre-dependent | DREADD | Roth |
135635 | pAAV-S5E2-Gq-P2A-dTomato | E2 regulatory element | Activator | DREADD | Dimidschstein |
Biosensors | |||||
104491 | pGP-AAV-syn-FLEX-jGCaMP7s-WPRE | Syn | Calcium sensor, Cre-dependent | GCaMP | Kim, GENIE |
104492 | pGP-AAV-syn-FLEX-jGCaMP7f-WPRE | Syn | Calcium sensor, Cre-dependent | GCaMP | Kim, GENIE |
104495 | pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE | CAG | Calcium sensor, Cre-dependent | GCaMP | Kim, GENIE |
104496 | pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE | CAG | Calcium sensor, Cre-dependent | GCaMP | Kim, GENIE |
104487 | pGP-AAV-syn-jGCaMP7s-WPRE | Syn | Calcium sensor | GCaMP | Kim, GENIE |
104488 | pGP-AAV-syn-jGCaMP7f-WPRE | Syn | Calcium sensor | GCaMP | Kim, GENIE |
135632 | pAAV-S5E2-GCaMP6f | E2 regulatory element | Calcium sensor | GCaMP | Dimidschstein |
100854 | pAAV.Syn.NES-jRGECO1a.WPRE.SV40 | Syn | Calcium sensor | RGECO | Kim, GENIE |
162375 | pGP-AAV-syn-jGCaMP8m-WPRE | Syn | Calcium sensor | GCaMP | GENIE |
Optogenetics | |||||
26973 | pAAV-hSyn-hChR2(H134R)-EYFP | Syn | Activator | ChR2 | Deisseroth |
20298 | pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA | EF1a | Activator, Cre-dependent | ChR2 | Deisseroth |
127090 | pAAV-CAG-DIO-ChR2(H134R)-eYFP | CAG | Activator, Cre-dependent | ChR2 | Gradinaru |
135633 | pAAV-S5E2-C1V1-eYFP | E2 regulatory element | Activator | C1V1 | Dimidschstein |
135634 | pAAV-S5E2-ChR2-mCherry | E2 regulatory element | Activator | ChR2 | Dimidschstein |
Recombinases | |||||
105540 | pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 | Syn | Cre-EGFP expression | Cre | Wilson |
105550 | pAAV.GFAP.Cre.WPRE.hGH | GFAP | Cre-expression | Cre | Wilson |
107738 | pAAV-hSyn-Cre-P2A-dTomato | Syn | Cre-expression | Cre | Larsen |
140135 | pAAV-EF1a-iCreV | EF1a | light-inducible recombinase | iCreV | Zeng |
140136 | pAAV-EF1a-iDreV | EF1a | light-inducible recombinase | iDreV | Zeng |
140137 | pAAV-EF1a-iFlpV | EF1a | light-inducible recombinase | iFlpV | Zeng |
164450 | CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA | minBG | iCre expression | iCre | Ting |
199775 | AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) | minBG | iCre(R297T) expression | iCre | AIBS, Ting |
199776 | AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) | minBG | iCre(R297T) expression | iCre | AIBS, Ting |
199777 | AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) | minBG | iCre(R297T) expression | iCre | AIBS, Ting |
PHP.S
These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006).
Browse Available PHP.S AAV
ID | Name | Promoter | Description | Category | PI |
---|---|---|---|---|---|
28306 | pAAV-FLEX-tdTomato | CAG | tdTomato, Cre-dependent | Control | Boyden |
59462 | pAAV-CAG-tdTomato | CAG | tdTomato | Control | Boyden |
PHP.V1
These viral vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847).
Browse Available PHP.V1 AAV
ID | Name | Promoter | Description | Category | PI |
---|---|---|---|---|---|
104052 | pAAV-CAG-DIO-EYFP | CAG | EYFP, Cre-dependent | Control | Gradinaru |
MaCPNS1
These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136).
Browse Available MaCPNS1 AAV
ID | Name | Promoter | Description | Category | PI |
---|---|---|---|---|---|
37825 | pAAV-CAG-GFP | CAG | GFP | Control | Boyden |
MaCPNS2
These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137).
Browse Available MaCPNS2 AAV
ID | Name | Promoter | Description | Category | PI |
---|---|---|---|---|---|
37825 | pAAV-CAG-GFP | CAG | GFP | Control | Boyden |
CAP-B10
These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004).
Browse Available CAP-B10 AAV
ID | Name | Promoter | Description | Category | PI |
---|---|---|---|---|---|
37825 | pAAV-CAG-GFP | CAG | GFP | Control | Boyden |
CAP-B22
These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid (Addgene #175005).
Browse Available CAP-B22 AAV
ID | Name | Promoter | Description | Category | PI |
---|---|---|---|---|---|
37825 | pAAV-CAG-GFP | CAG | GFP | Control | Boyden |
AAV9-X1.1
These viral vector preparations were produced with the pUCmini-iCAP-AAV9-X1.1 plasmid (Addgene #196836).
Browse Available AAV9-X1.1
ID | Name | Promoter | Description | Category | PI |
---|---|---|---|---|---|
37825 | pAAV-CAG-GFP | CAG | GFP | Control | Boyden |
Citation Information
When using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows:
- PHP.eB, PHP.S: Chan et al., 2017 Pubmed 28671695 (Link opens in a new window)
- PHP.V1: Kumar et al., 2020 Pubmed 32313222 (Link opens in a new window)
- MaCPNS1, MaCPNS2: Chen et al., 2022 Pubmed 35643078 (Link opens in a new window)
- CAP-B10, CAP-B22: Goertsen, Flytzanis, et al., 2022 Pubmed 34887588 (Link opens in a new window)
- AAV9-X1.1: Chen et al., 2023 Pubmed 37291094 (Link opens in a new window)
Don’t See What You’re Looking For?
Please let us know (Link opens in a new window) what you would like to see available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.
Text Description for the AAV Decision Tree
The AAV decision tree graphic above helps you choose the best capsid for your cell type. First, do you want to target CNS or PNS? Then consider species, and then cell type.
For CNS:
- For infant rhesus macaques:
- Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2
- Cell type-biased:
- Neurons - AAV.CAP-Mac, AAV9-X1.1
- For marmosets:
- Broad tropism - AAV.CAP-B22
- Cell type-biased:
- Neurons - AAV.CAP-B10
- Brain vascular cells - AAV.CAP-Mac
- For rats:
- Broad tropism - AAV-PHP.eB
- Cell type-biased:
- Neurons - AAV.CAP-Mac
- Brain vascular cells - AAV9-X1.1, AAV1-X1
- For mice:
- Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC
- Cell type-biased:
- Astrocytes - AAV-PHP.AX with GRE or transgenic animal
- Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal
- Brain vascular cells - AAV9-X1.1, AAV1-X1
- Neurons - AAV.CAP-B10, AAV-PHP.N
- Broad - AAV-PHP.AX with GRE or transgenic animal
- L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal
- L2/3 & L4 - AAV.CAP-B10 with GRE or transgenic animal
- Excitatory - AAV.PHP.N with GRE or transgenic animal
For PNS applications in rodents and NHPs:
- Broad tropism - AAV-MaCPNS2, AAV-PHP.S
- Sensory nervous system - AAV-MaCPNS1