Viral Vector Packaging Service: Molecular Tools
Tetracycline Transactivators and Inducible Tools
These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression systems.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 99118 | pAAV-CAG-tTA | CAG-driven, constitutive | Expression of the tet-off transactivator (tTA) | 2 | Gradinaru |
| 99120 | pAAV-ihSyn1-tTA | Inducible Synapsin promoter (ihSyn) | Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. | 1 | Gradinaru |
| 99121 | pAAV-ihSyn1-DIO-tTA | Cre-dependent and inducible Synapsin promoter (ihSyn) | Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. | 1 | Gradinaru |
| 117383 | TRE-DIO-eYFP | Cre-dependent and Tetracycline-inducible | Cre-dependent, Tet-inducible expression of EYFP | 1 | Gradinaru |
Tools for Affinity Purification
These AAV encode tools for affinity purification (which can be used for viral TRAP).
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 98747 | pAAV-FLEX-EGFPL10a | EF1a-driven and Cre-dependent | EGFP-tagged ribosomal L10a | 5, rg* | Heintz, Nectow, Schmidt |
Neurophysiology Tools
These AAV encode tools that can be used for neurophysiology experiments, including neuronal connectivity labeling.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 51509 | AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE | Syn-driven, Cre-dependent | Cre-dependent expression of cytoplasmic tdTomato and synaptophysin-EGFP for labeling of axon terminals. | 1 | Zeng |
| 71760 | pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby | Syn-driven, Cre-dependent | Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling of axon terminals. | 1 | Luo |
| 60658 | pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato | EF1a-driven, Flp-dependent | Flp-dependent expression of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato | 8 | Scanziani |
Tools for Cell Ablation
These AAV encode tools that can be used for cell ablation.
| ID | Name | Expression System | Activity | Serotype | PI |
|---|---|---|---|---|---|
| 124364 | pAAV-FLEX-DTR-GFP | CBA-driven, Cre-dependent | Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation. | 2 | Jessell, Azim |
| 45580 | pAAV-flex-taCasp3-TEVp | EF1a-driven, Cre-dependent | Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. | 5 | Shah, Wells |
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