Viral Vector Packaging Service: Caltech Systemic Capsids

As part of our Viral Vector Packaging Service, Addgene is distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in a new window). As part of our standard viral production, these viral vectors undergo quality control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation.

Systemic Delivery AAV Capsid Choice

Need help deciding which capsid to choose? The following decision tree was developed at the CLOVER Center at Caltech and can help guide your decision.

AAV decision tree. Described under the heading Text Description for the AAV Decision Tree — Figure 1: Decision tree for choosing your AAV capsid. Your choice could depend on several factors including: the delivery method, mouse strain, target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang, et al., 2019) for LY6A-permissive mouse strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center (Link opens in a new window)and was adapted from Challis et al. (2022). Text version of the AAV decision tree can be found at the bottom of this page.

For more in depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids. To review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository of published capsid use (Link opens in a new window).

Viral Vectors 101: Systemic Capsids

PHP.eB

These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005).

Note on PHP.eB tropism and specific mouse lines: Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (Huang et al., 2019 (Link opens in a new window)) to cross the blood brain barrier. For information on whether the PHP.eB serotype will work in your mouse line, see Supplementary Table 3 (Link opens in a new window).

Browse Available PHP.eB AAV

ID	Name	Promoter	Description	Category	PI
Controls
28306	pAAV-FLEX-tdTomato	CAG	tdTomato, Cre-dependent	Control	Boyden
37825	pAAV-CAG-GFP	CAG	GFP	Control	Boyden
50469	pAAV-CaMKIIa-EGFP	CamKIIa	EGFP	Control	Roth
59462	pAAV-CAG-tdTomato	CAG	tdTomato	Control	Boyden
83900	pAAV-mDlx-GFP-Fishell-1	Dlx	GFP	Control	Fishell
99130	pAAV-mDlx-NLS-mRuby2	Dlx	mRuby2	Control	Gradinaru
104055	pAAV-CAG-eYFP	CAG	EYFP	Control	Gradinaru
104061	CAG-NLS-GFP	CAG	NLS-GFP	Control	Gradinaru
105530	pAAV.CMV.PI.EGFP.WPRE.bGH	CMV	EGFP	Control	Wilson
105547	pENN.AAV.EF1a.eGFP.WPRE.rBG	EF1a	EGFP	Control	Wilson
114469	pAAV-CaMKIIa-mCherry	CamKIIa	mCherry	Control	Deisseroth
114470	pAAV-Ef1a-mCherry	EF1a	mCherry	Control	Deisseroth
117382	hSyn1-eYFP	Syn	EYFP	Control	Gradinaru
135630	pAAV-S5E2-dTom-nlsdTom	E2 regulatory element	dTomato	Control	Dimidschstein
135631	pAAV-S5E2-GFP-fGFP	E2 regulatory element	GFP, membrane-targeted GFP	Control	Dimidschstein
192552	pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA	CAG	tdTomato	Control	Feng
163505	CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA	minBetaGlobin	SYFP2	Control	Ting
163509	CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA	hSyn1	H2B-SYFP2	Control	Ting
191706	AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044)	minBG	SYFP2	Control	AIBS, Ting
191720	AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038)	minBG	SYFP2	Control	AIBS, Ting
191728	AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408)	minBG	SYFP2	Control	AIBS, Ting
199773	AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863)	minBG	SYFP2	Control	AIBS, Ting
199774	AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905)	minBG	SYFP2	Control	AIBS, Ting
Chemogenetics
44361	pAAV-hSyn-DIO-hM3D(Gq)-mCherry	Syn	Activator, Cre-dependent	DREADD	Roth
44362	pAAV-hSyn-DIO-hM4D(Gi)-mCherry	Syn	Inhibitor, Cre-dependent	DREADD	Roth
135635	pAAV-S5E2-Gq-P2A-dTomato	E2 regulatory element	Activator	DREADD	Dimidschstein
Biosensors
104491	pGP-AAV-syn-FLEX-jGCaMP7s-WPRE	Syn	Calcium sensor, Cre-dependent	GCaMP	Kim, GENIE
104492	pGP-AAV-syn-FLEX-jGCaMP7f-WPRE	Syn	Calcium sensor, Cre-dependent	GCaMP	Kim, GENIE
104495	pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE	CAG	Calcium sensor, Cre-dependent	GCaMP	Kim, GENIE
104496	pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE	CAG	Calcium sensor, Cre-dependent	GCaMP	Kim, GENIE
104487	pGP-AAV-syn-jGCaMP7s-WPRE	Syn	Calcium sensor	GCaMP	Kim, GENIE
104488	pGP-AAV-syn-jGCaMP7f-WPRE	Syn	Calcium sensor	GCaMP	Kim, GENIE
135632	pAAV-S5E2-GCaMP6f	E2 regulatory element	Calcium sensor	GCaMP	Dimidschstein
100854	pAAV.Syn.NES-jRGECO1a.WPRE.SV40	Syn	Calcium sensor	RGECO	Kim, GENIE
162375	pGP-AAV-syn-jGCaMP8m-WPRE	Syn	Calcium sensor	GCaMP	GENIE
Optogenetics
26973	pAAV-hSyn-hChR2(H134R)-EYFP	Syn	Activator	ChR2	Deisseroth
20298	pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA	EF1a	Activator, Cre-dependent	ChR2	Deisseroth
127090	pAAV-CAG-DIO-ChR2(H134R)-eYFP	CAG	Activator, Cre-dependent	ChR2	Gradinaru
135633	pAAV-S5E2-C1V1-eYFP	E2 regulatory element	Activator	C1V1	Dimidschstein
135634	pAAV-S5E2-ChR2-mCherry	E2 regulatory element	Activator	ChR2	Dimidschstein
Recombinases
105540	pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40	Syn	Cre-EGFP expression	Cre	Wilson
105550	pAAV.GFAP.Cre.WPRE.hGH	GFAP	Cre-expression	Cre	Wilson
107738	pAAV-hSyn-Cre-P2A-dTomato	Syn	Cre-expression	Cre	Larsen
140135	pAAV-EF1a-iCreV	EF1a	light-inducible recombinase	iCreV	Zeng
140136	pAAV-EF1a-iDreV	EF1a	light-inducible recombinase	iDreV	Zeng
140137	pAAV-EF1a-iFlpV	EF1a	light-inducible recombinase	iFlpV	Zeng
164450	CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA	minBG	iCre expression	iCre	Ting
199775	AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778)	minBG	iCre(R297T) expression	iCre	AIBS, Ting
199776	AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779)	minBG	iCre(R297T) expression	iCre	AIBS, Ting
199777	AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)	minBG	iCre(R297T) expression	iCre	AIBS, Ting

PHP.S

These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006).

Browse Available PHP.S AAV

ID	Name	Promoter	Description	Category	PI
28306	pAAV-FLEX-tdTomato	CAG	tdTomato, Cre-dependent	Control	Boyden
59462	pAAV-CAG-tdTomato	CAG	tdTomato	Control	Boyden

PHP.V1

These viral vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847).

Browse Available PHP.V1 AAV

ID	Name	Promoter	Description	Category	PI
104052	pAAV-CAG-DIO-EYFP	CAG	EYFP, Cre-dependent	Control	Gradinaru

MaCPNS1

These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136).

Browse Available MaCPNS1 AAV

ID	Name	Promoter	Description	Category	PI
37825	pAAV-CAG-GFP	CAG	GFP	Control	Boyden

MaCPNS2

These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137).

Browse Available MaCPNS2 AAV

ID	Name	Promoter	Description	Category	PI
37825	pAAV-CAG-GFP	CAG	GFP	Control	Boyden

CAP-B10

These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004).

Browse Available CAP-B10 AAV

ID	Name	Promoter	Description	Category	PI
37825	pAAV-CAG-GFP	CAG	GFP	Control	Boyden

CAP-B22

These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid (Addgene #175005).

Browse Available CAP-B22 AAV

ID	Name	Promoter	Description	Category	PI
37825	pAAV-CAG-GFP	CAG	GFP	Control	Boyden

AAV9-X1.1

These viral vector preparations were produced with the pUCmini-iCAP-AAV9-X1.1 plasmid (Addgene #196836).

Browse Available AAV9-X1.1

ID	Name	Promoter	Description	Category	PI
37825	pAAV-CAG-GFP	CAG	GFP	Control	Boyden

Citation Information

When using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows:

PHP.eB, PHP.S: Chan et al., 2017 Pubmed 28671695 (Link opens in a new window)
PHP.V1: Kumar et al., 2020 Pubmed 32313222 (Link opens in a new window)
MaCPNS1, MaCPNS2: Chen et al., 2022 Pubmed 35643078 (Link opens in a new window)
CAP-B10, CAP-B22: Goertsen, Flytzanis, et al., 2022 Pubmed 34887588 (Link opens in a new window)
AAV9-X1.1: Chen et al., 2023 Pubmed 37291094 (Link opens in a new window)

Don’t See What You’re Looking For?

Please let us know (Link opens in a new window) what you would like to see available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.

Text Description for the AAV Decision Tree

The AAV decision tree graphic above helps you choose the best capsid for your cell type. First, do you want to target CNS or PNS? Then consider species, and then cell type.

For CNS:

For infant rhesus macaques:
- Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2
- Cell type-biased:
  - Neurons - AAV.CAP-Mac, AAV9-X1.1
For marmosets:
- Broad tropism - AAV.CAP-B22
- Cell type-biased:
  - Neurons - AAV.CAP-B10
  - Brain vascular cells - AAV.CAP-Mac
For rats:
- Broad tropism - AAV-PHP.eB
- Cell type-biased:
  - Neurons - AAV.CAP-Mac
  - Brain vascular cells - AAV9-X1.1, AAV1-X1
For mice:
- Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC
- Cell type-biased:
  - Astrocytes - AAV-PHP.AX with GRE or transgenic animal
  - Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal
  - Brain vascular cells - AAV9-X1.1, AAV1-X1
  - Neurons - AAV.CAP-B10, AAV-PHP.N
    - Broad - AAV-PHP.AX with GRE or transgenic animal
    - L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal
    - L2/3 & L4 - AAV.CAP-B10 with GRE or transgenic animal
    - Excitatory - AAV.PHP.N with GRE or transgenic animal

For PNS applications in rodents and NHPs:

Broad tropism - AAV-MaCPNS2, AAV-PHP.S
Sensory nervous system - AAV-MaCPNS1

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