Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pM
Information
- Source/Vendor
- Clontech
- Alt Name
- pM DNA-BD
- Promoter
- SV40 early promoter
- Expression Level
- Unknown
- Cloning Method
- Restriction Enzyme
- Size
- 3454
- Bacterial Resistance
- Ampicillin
- Notes
- Description from Clontech: "pM is used to generate a fusion of the GAL4 DNA-BD (amino acids 1–147) and a protein of interest in the Matchmaker™ Mammalian Assay Kit 2 (Cat. No. 630305). The hybrid protein is targeted to the cell’s nucleus by the GAL4 nuclear localization sequence. Genes encoding test proteins should be cloned, in the correct orientation and reading frame, into one of the unique restriction sites in the MCS region at the 3’ end of the GAL4 DNA-BD. Restriction sites marked with an (*) are in the same reading frame as pGBKT7. Transcription is initiated from the constitutive SV40 early promoter (PSV40e); transcription is terminated at the SV40 poly A transcription termination signal."
- Catalog Number
- PT3119-5
- Stable
- Transient
- Constitutive
- Constitutive
- Viral/Non-Viral
- Nonviral