Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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This vector is not available from Addgene.

Plasmid: pmirGLO

Information

Source/Vendor

Promega

Plasmid Type

Dual-Luciferase miRNA Target Expression Vector

Promoter

PGK, SV40

Expression Level

Unknown

Cloning Method

Restriction Enzyme

Size

7350

5' Sequencing 1 Primer

SV40pro-F2

5' Sequencing 1 Primer Sequence

CCCCATGGCTGACTAATTTTT

3' Sequencing 1 Primer

Neo-R

3' Sequencing 1 Primer Sequence

GCCCAGTCATAGCCGAATAG

5' Sequencing 2 Primer

mPGK-F

5' Sequencing 2 Primer Sequence

cattctgcacgcttcaaaag

3' Sequencing 2 Primer

EBV-rev

3' Sequencing 2 Primer Sequence

GTGGTTTGTCCAAACTCATC

5' Terminal

N-Term

5' Terminal 2

N-Term

3' Terminal

C-Term

3' Terminal 2

C-Term

GenBank

FJ376737.1

Stable

Unspecified

Constitutive

Unspecified

Viral/Non-Viral

Nonviral

Sequence

Copy sequence

CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG 51 CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT 101 TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 151 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT 201 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG 251 CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT 351 AACGCTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG 401 TATTTCACAC CGCATACGCG GATCTGCGCA GCACCATGGC CTGAAATAAC 451 CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA AAGAACCAGC 501 TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 551 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 651 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 701 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 751 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT 801 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGATTCT 851 TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG 951 GGCTCGCTGC AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG 1001 ATTCCGAGAA GCACGCCGAG AACGCCGTGA TTTTTCTGCA TGGTAACGCT 1051 GCCTCCAGCT ACCTGTGGAG GCACGTCGTG CCTCACATCG AGCCCGTGGC 1101 TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC GGCAAGAGCG 1151 GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG 1251 GGGGGCTTGT CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA 1301 AGGCCATCGT CCATGCTGAG AGTGTCGTGG ACGTGATCGA GTCCTGGGAC 1351 GAGTGGCCTG ACATCGAGGA GGATATCGCC CTGATCAAGA GCGAAGAGGG 1401 CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC ATGCTCCCAA 1451 GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG 1551 CGAGATCCCT CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC 1601 GCAACTACAA CGCCTACCTT CGGGCCAGCG ACGATCTGCC TAAGATGTTC 1651 ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC GCTATTGTCG AGGGAGCTAA 1701 GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC CACTTCAGCC 1751 AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC 1851 AGGTGGCGGA GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT 1901 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 1951 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG 2001 CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC 2051 AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT 2151 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG 2201 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 2251 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC 2301 ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG 2351 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC 2451 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC 2501 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 2551 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA 2601 CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG 2651 ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT 2751 ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGCGATA AGGATCCTCT 2801 TTGCGCTTGC GTTTTCCCTT GTCCAGATAG CCCAGTAGCT GACATTCATC 2851 CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACGTAA TGGTTTCTTA 2901 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 2951 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 3001 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA 3051 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 3101 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG 3151 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 3201 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTCAAAG 3251 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 3301 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC 3351 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 3401 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA 3451 ACTATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 3501 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 3551 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 3601 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT 3651 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 3701 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT 3751 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 3801 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 3851 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAATTC 3901 GAAATGACCG ACCAAGCGAC GCCCAACCGG TATCAGCTCA CTCAAAGGCG 3951 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 4001 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 4051 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 4101 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 4151 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 4201 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 4251 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 4301 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 4351 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 4401 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 4451 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG 4501 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 4551 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 4601 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATTT CAAGAAGATC 4651 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 4701 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 4751 TTTATAGTCC GGAAATACAG GAACGCACGC TGGATGGCCC TTCGCTGGGA 4801 TGGTGAAACC ATGAAAAATG GCAGCTTCAG TGGATTAAGT GGGGGTAATG 4851 TGGCCTGTAC CCTCTGGTTG CATAGGTATT CATACGGTTA AAATTTATCA 4901 GGCGCGATTG CGGCAGTTTT TCGGGTGGTT TGTTGCCATT TTTACCTGTC 4951 TGCTGCCGTG ATCGCGCTGA ACGCGTTTTA GCGGTGCGTA CAATTAAGGG 5001 ATTATGGTAA ATCCACTTAC TGTCTGCCCT CGTAGCCATC GAGATAAACC 5051 GCAGTACTCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTACCGG 5101 GTAGGGGAGG CGCTTTTCCC AAGGCAGTCT GGAGCATGCG CTTTAGCAGC 5151 CCCGCTGGGC ACTTGGCGCT ACACAAGTGG CCTCTGGCCT CGCACACATT 5201 CCACATCCAC CGGTAGGCGC CAACCGGCTC CGTTCTTTGG TGGCCCCTTC 5251 GCGCCACCTT CTACTCCTCC CCTAGTCAGG AAGTTCCCCC CCGCCCCGCA 5301 GCTCGCGTCG TGCAGGACGT GACAAATGGA AGTAGCACGT CTCACTAGTC 5351 TCGTGCAGAT GGACAGCACC GCTGAGCAAT GGAAGCGGGT AGGCCTTTGG 5401 GGCAGCGGCC AATAGCAGCT TTGCTCCTTC GCTTTCTGGG CTCAGAGGCT 5451 GGGAAGGGGT GGGTCCGGGG GCGGGCTCAG GGGCGGGCTC AGGGGCGGGG 5501 CGGGCGCCCG AAGGTCCTCC GGAGGCCCGG CATTCTGCAC GCTTCAAAAG 5551 CGCACGTCTG CCGCGCTGTT CTCCTCTTCC TCATCTCCGG GCCTTTCGAC 5601 CTGCAGCCCA AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGAA 5651 GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC CACTCGAAGA 5701 CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG 5751 TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC 5801 TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG 5851 CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT 5901 TGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG 5951 GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG 6001 CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA 6051 TCCTCAACGT GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG 6101 GATAGCAAGA CCGACTACCA GGGCTTCCAA AGCATGTACA CCTTCGTGAC 6151 TTCCCATTTG CCACCCGGCT TCAACGAGTA CGACTTCGTG CCCGAGAGCT 6201 TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGCAGTACC 6251 GGATTGCCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT 6301 CAGTCATGCC CGCGACCCCA TCTTCGGCAA CCAGATCATC CCCGACACCG 6351 CTATCCTCAG CGTGGTGCCA TTTCACCACG GCTTCGGCAT GTTCACCACG 6401 CTGGGCTACT TGATCTGCGG CTTTCGGGTC GTGCTCATGT ACCGCTTCGA 6451 GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA CTATAAGATT CAATCTGCCC 6501 TGCTGGTGCC CACACTATTT AGCTTCTTCG CTAAGAGCAC TCTCATCGAC 6551 AAGTACGACC TAAGCAACTT GCACGAGATC GCCAGCGGCG GGGCGCCGCT 6601 CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA ACGCTTCCAC CTACCAGGCA 6651 TCCGCCAGGG CTACGGCCTG ACAGAAACAA CCAGCGCCAT TCTGATCACC 6701 CCCGAAGGGG ACGACAAGCC TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT 6751 CGAGGCTAAG GTGGTGGACT TGGACACCGG TAAGACACTG GGTGTGAACC 6801 AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA TGATCATGAG CGGCTACGTT 6851 AACAACCCCG AGGCTACAAA CGCTCTCATC GACAAGGACG GCTGGCTGCA 6901 CAGCGGCGAC ATCGCCTACT GGGACGAGGA CGAGCACTTC TTCATCGTGG 6951 ACCGGCTGAA GAGCCTGATC AAATACAAGG GCTACCAGGT AGCCCCAGCC 7001 GAACTGGAGA GCATCCTGCT GCAACACCCC AACATCTTCG ACGCCGGGGT 7051 CGCCGGCCTG CCCGACGACG ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG 7101 TGCTGGAACA CGGTAAAACC ATGACCGAGA AGGAGATCGT GGACTATGTG 7151 GCCAGCCAGG TTACAACCGC CAAGAAGCTG CGCGGTGGTG TTGTGTTCGT 7201 GGACGAGGTG CCTAAAGGAC TGACCGGCAA GTTGGACGCC CGCAAGATCC 7251 GCGAGATTCT CATTAAGGCC AAGAAGGGCG GCAAGATCGC CGTGTAATTC 7301 TAGTTGTTTA AACGAGCTCG CTAGCCTCGA GTCTAGAGTC GACCTGCAGG