Vector Database

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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

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Plasmid: pHTN HaloTag CMV-neo

Information

Source/Vendor

Promega

Plasmid Type

Mammalian Expression

Promoter

CMV

Expression Level

Unknown

Cloning Method

Restriction Enzyme

Size

6143

5' Sequencing 1 Primer

T7

5' Sequencing 1 Primer Sequence

TAATACGACTCACTATAGGG

3' Sequencing 1 Primer

EBV-rev

3' Sequencing 1 Primer Sequence

GTGGTTTGTCCAAACTCATC

5' Terminal

N-Term

Tag 1

HALO tag

Bacterial Resistance

Ampicillin

Selectable Marker

Neomycin

Stable

Unspecified

Constitutive

Constitutive

Viral/Non-Viral

Nonviral

Sequence

Copy sequence

1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCAAA GCCACCATGG CAGAAATCGG TACTGGCTTT CCATTCGACC 1101 CCCATTATGT GGAAGTCCTG GGCGAGCGCA TGCACTACGT CGATGTTGGT 1151 CCGCGCGATG GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC 1201 CTACGTGTGG CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA 1251 TTGCTCCAGA CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT 1301 TATTTCTTCG ACGACCACGT CCGCTTCATG GATGCCTTCA TCGAAGCCCT 1351 GGGTCTGGAA GAGGTCGTCC TGGTCATTCA CGACTGGGGC TCCGCTCTGG 1401 GTTTCCACTG GGCCAAGCGC AATCCAGAGC GCGTCAAAGG TATTGCATTT 1451 ATGGAGTTCA TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC 1501 CCGCGAGACC TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA 1551 TCATCGATCA GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC 1601 CGCCCGCTGA CTGAAGTCGA GATGGACCAT TACCGCGAGC CGTTCCTGAA 1651 TCCTGTTGAC CGCGAGCCAC TGTGGCGCTT CCCAAACGAG CTGCCAATCG 1701 CCGGTGAGCC AGCGAACATC GTCGCGCTGG TCGAAGAATA CATGGACTGG 1751 CTGCACCAGT CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT 1801 TCTGATCCCA CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT 1851 GCAAGGCTGT GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC 1901 CCGGACCTGA TCGGCAGCGA GATCGCGCGC TGGCTGTCGA CGCTCGAGAT 1951 TTCCGGCGAG CCAACCACTG AGGATCTGTA CTTTCAGAGC GATAACGCGA 2001 TCGCTTCCGA ATTCAGAGCT CAACCGCGGA TATCTAGATT TGGGCCCAAT 2051 TCCTGCAGGA TTTTGCGGCC GCTTGCTGAG TTGGCTGCTG CCACCGCTGA 2101 GCAATAACTA GCATAACCCC TTGGCCGCTT CGAGCAGACA TGATAAGATA 2151 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT 2201 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC 2251 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT 2301 TCAGGGGGAG ATGTGGGAGG TTTTTTTAAG CAAGTAAAAC CTCTACAAAT 2351 GTGGTAAAAT CGAATTTTAA CAAAATATTA ACGCTTACAA TTTCCTGATG 2401 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGCGG 2451 ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG GAACTTGGTT 2501 AGGTACCTTC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG 2551 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 2601 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC 2651 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC 2701 CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 2751 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC 2801 CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 2851 GCCTAGGCTT TTGCAAAAAG CTTGATTCTT CTGACACAAC AGTCTCGAAC 2901 TTAAGGCTAG AGCCACCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 2951 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC 3001 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC 3051 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 3101 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 3151 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG 3201 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 3251 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 3301 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC 3351 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 3401 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 3451 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA 3501 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 3551 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT 3601 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG 3651 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 3701 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 3751 CCCAACCTGC CATCACGATG GCCGCAATAA AATATCTTTA TTTTCATTAC 3801 ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGCGATAAG GATCCTCTTT 3851 GCGCTTGCGT TTTCCCTTGT CCAGATAGCC CAGTAGCTGA CATTCATCCG 3901 GGGTCAGCAC CGTTTCTGCG GACTGGCTTT CTACGTAATG GTTTCTTAGA 3951 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 4001 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG 4051 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 4101 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 4151 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 4201 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG 4251 AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTCAAAGTT 4301 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT 4351 CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 4401 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 4451 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 4501 TATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC 4551 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 4601 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG 4651 CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 4701 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 4751 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 4801 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA 4851 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 4901 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAATTCGA 4951 AATGACCGAC CAAGCGACGC CCAACCGGTA TCAGCTCACT CAAAGGCGGT 5001 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 5051 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG 5101 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 5151 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 5201 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 5251 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 5301 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 5351 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT 5401 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 5451 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 5501 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA 5551 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 5601 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 5651 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATTTCA AGAAGATCCT 5701 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 5751 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 5801 TATAGTCCGG AAATACAGGA ACGCACGCTG GATGGCCCTT CGCTGGGATG 5851 GTGAAACCAT GAAAAATGGC AGCTTCAGTG GATTAAGTGG GGGTAATGTG 5901 GCCTGTACCC TCTGGTTGCA TAGGTATTCA TACGGTTAAA ATTTATCAGG 5951 CGCGATTGCG GCAGTTTTTC GGGTGGTTTG TTGCCATTTT TACCTGTCTG 6001 CTGCCGTGAT CGCGCTGAAC GCGTTTTAGC GGTGCGTACA ATTAAGGGAT 6051 TATGGTAAAT CCACTTACTG TCTGCCCTCG TAGCCATCGA GATAAACCGC 6101 AGTACTCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCT