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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pPFHW

Information

Source/Vendor
Drosophila Genomics Resource Center
Plasmid Type
Insect Expression
Promoter
UASp
Expression Level
Unknown
Cloning Method
Gateway Cloning
Size
11908
5' Terminal
N-Term
5' Terminal 2
N-Term
Tag 1
3xFLAG
Tag 2
3xHA
Bacterial Resistance
Ampicillin and Chloramphenicol
Notes
vector: pPFHW UASp / 3xFLAG-3xHA / N-terminus features: UASp promoter: 4763..513 3xFLAG-3xHA: 5518..5742 Gateway cassette: 5752..7455 attR1: 5752..5769 attR2: 7438..7455 triple stop: 7455..7467 K10 terminator: 7519..8902 ampR: 10055..10915 chlR: 5985..6665 ccdB: 6985..7290 mini-white (complement): 1364..4244 P 5Õ end: 1..587 P 3Õ end (complement): 8916..9148 ------
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Nonviral

Sequence