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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pAFHW

Information

Source/Vendor
Drosophila Genomics Resource Center
Plasmid Type
Insect Expression
Promoter
Actin5C
Expression Level
Unknown
Cloning Method
Gateway Cloning
Size
7465
5' Terminal
N-Term
5' Terminal 2
N-Term
Tag 1
3xFLAG
Tag 2
3xHA
Bacterial Resistance
Ampicillin and Chloramphenicol
Notes
vector: pAFHW Actin5C / 3xFLAG-3xHA / N-terminus features: Actin5C promoter: 90..2662 3xFLAG-3xHA: 2764..2988 Gateway cassette: 2998..4701 attR1: 2998..3015 attR2: 4684..4701 triple stop: 4701..4713 SV40 sti/polyA: 4725..5567 ampR: 5664..6524 chlR: 3231..3911 ccdB: 4231..4536
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Nonviral

Sequence