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Vector Database


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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pCDFDuet-1

Information

Source/Vendor
Novagen (EMD Millipore)
Alt Name
pCDFDuet™-1
Plasmid Type
Bacterial Expression
Promoter
AmpR
Expression Level
Unknown
Cloning Method
Restriction Enzyme
Size
3781
5' Sequencing 1 Primer
ACYCDuetUP1
5' Sequencing 1 Primer Sequence
GGATCTCGACGCTCTCCCT
3' Sequencing 1 Primer
DuetDOWN1
3' Sequencing 1 Primer Sequence
GATTATGCGGCCGTGTACAA
5' Sequencing 2 Primer
DuetUP2
5' Sequencing 2 Primer Sequence
TTGTACACGGCCGCATAATC
3' Sequencing 2 Primer
T7term
3' Sequencing 2 Primer Sequence
GCTAGTTATTGCTCAGCGG
5' Terminal
N-Term
5' Terminal 2
N-Term
3' Terminal
C-Term
3' Terminal 2
C-Term
Tag 1
6xHis
Tag 2
S15
Bacterial Resistance
Streptomycin
Notes
pCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Nonviral

Sequence