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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: YRp7-32phocat

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
6530
Notes
A 570 bp SalI/EcoRI fragment containing the PHO5 promoter was ligated to a 5965 bp BamHI/SalI fragment of YRp7-32cat (ATCC 37624). The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al. One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast. Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 1.37, 0.55; PstI--5.5, 1.3; PvuII--4.7, 2.15. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli RR1, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37625
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified