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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: YRp14/ARS1/CEN4

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
11500
Notes
YC-type shuttle plasmid which can be used as a reporter of chromosome segregation due to loss of SUP11-containing chromosomes. Constructed by inserting a 0.8 kb EcoRI/HindIII fragment containing ARS1 and a 6.0 kb BamHI/SalI fragment containing CEN4 into the corresponding sites of YRp14. Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.1, 2.3, 1.6, 0.8, 0.7; BamHI--11.5; EcoRI--5.5, 4.5, 1.5; SalI--11.5. (ATCC staff) The order of the major features in this plasmid is: ARS1 - BamHI - CEN4 - SalI - URA3 - SUP11 - rep - pMB1 ori - bla. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. Related vectors: YRp14/ARS1 from YRp14 and ARS1, YRp14/HIS3, YRp14/TRP1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77161
GenBank
L22015, M11994, M11997, M11995, M11996, M11998, M11999
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified