Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: YRp14-his3delta200
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 11200
- Notes
- A 1.0 kb region that encompasses the entire coding region of HIS3 was deleted from a genomic EcoRI/SalI fragment (final size=5.1 kb) cloned into the EcoRI/SalI sites of YRp14. [1] A marker addition/transplacement vector. [1] Restriction digests of the clone give the following sizes (kb): BamHI--11.0,0.5; PstI--6.1, 4.0, 1.2; BglII--10.0, 1.15; KpnI--11.5. (ATCC staff) Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77146
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified