Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: YpGX265GAL4
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 12300
- Notes
- High copy number YE-type shuttle expression vector. [1] The promoter consists of nt -610 to -157 of the GAL 1-10 upstream activation site,fused to the transcription initiation site (nt -158 to -1) of the alpha mating factor gene MFalpha1. [1] The 3.65 kb HindIII fragment encodes the positive regulator protein GAL4, necessary for transcription from the GAL1-10 promoter. [1] The PHO5 signal sequence (51 nt) was modified to use preferred Saccharomyces codons. [1] The plasmid is maintained at approximately 200 copies per cell in haploid S. cerevisiae cells. [1] The order of the major features in the plasmid is: HindIII - GAL4 - HindIII - Gal1-10 UAS - MFalpha1 transcription initiation sequence - PHO5 signal sequence - EcoRV - BamHI - GAPDH terminator - ampR - LEU2-d - 2 micron ori. [1] Restriction digests of the clone give the following sizes (kb): EcoRV--5.1, 4.2, 3.0; BamHI--8.1, 4.2; HindIII--8.5, 3.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, Saccharomyces cerevisiae, S.cerevisiae 442, S.cerevisiae SHY1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 67233
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified