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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: YIp29

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
7800
Notes
A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. Unique restriction sites are in the 3' untranslated regions of URA3(SmaI) and LEU2(SalI). [2] Orientations are such that transcription from both URA3 and LEU2 is clockwise. [2] A general purpose integrating Saccharomyces cerevisiae/E.coli shuttle vector. [1] Restriction digests analyzed on agarose gels give the following sizes (kb): EcoRI--4.7, 3.2; BamHI--7.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli MM294, E.coli C600, S.cerevisiae SHY series. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37054
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified