Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: YEp366R
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 8400
- Notes
- Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; PstI--8.4. (ATCC staff) The sequence and reading frame of the multiple cloning sequence is: 5' AAG CTT GCA TGC CTG CAG GTC GAC TCT AGA GGA TCC CCG GGT ACC GAG CTC GAA TTC CCA GCT TGC GAT CCC 3', from nucleotide 1 of MCS through CCC for amino acid 8 of beta-galactosidase. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37740 to ATCC 37742) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-1; SphI-2; PstI-2; SalI-1; XbaI-1; BamHI-1; SmaI-2; KpnI-2; SacI-2; EcoRI-1. The EcoRI, KpnI, and SacI sites are not unique. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, E.coli MC1061. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37740
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified