Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: YEp351
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5644
- Notes
- pUC18 was digested with NdeI and blunt-ended by filling in. A 2.95 BamHI/XbaI fragment of pC4B was blunt-ended (filled in) and ligated to the pUC18. The 2.95 kb fragment of pC4B includes the LEU2 gene (nt 286-2256 in the final construct, where the A of the ATG of lacZ' is number 1) and the 2 micron replication system (nt 2257-3241). Restriction digests of the clone give the following sizes (kb): BamHI--6.0; XbaI--6.0; PstI--6.0; HindIII--6.0. (ATCC staff) This is a E.coli/yeast episomal shuttle vector with multiple restriction sites for cloning. (personal communication) Medium is 1227 LB plus ampicillin. NCBI gi: 295270 Hosts: E.coli RR1, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pBR322, 2micron. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)
- Catalog Number
- 37672
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified