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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: YCF4

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
8500
Notes
Shuttle chromosome fragmentation vector. [1] Constructed by inserting a 1.4 kb fragment containing CEN4 at the SalI site (blunt-ended) of YCF3. [1] The order of the major features in this plasmid is: Y' - MCS - CEN4 - URA3 - SUP11 - rep - pMB1 ori - bla. [1] The sequence to be mapped is cloned into the MCS of both YCF3 (ATCC 77155) and YCF4 (ATCC 77156) (both orientations being produced). NotI is used to linearize the constructs, which are transformed independently into YPH49. [2] YCF4 is used to recover sequences distal to the mapping sequence. [2] Restriction digests of the clone give the following sizes (kb): EcoRI--8.5; HindIII--8.5; BamHI--8.5; SalI (uncut). (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH49. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77156
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified