Skip to main content
Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pWT551

Information

Plasmid Type
Unspecified
Cloning Method
Unknown
Size
3773
Notes
Assembled from pAT153 and GenBank:EcoTrpPO according to the publication cited by F. Pfeiffer. There is confliciting information on the structure of pAT153. some 200 bases may be erraneuosly missing from this sequence. The construction of three different types of trp promoter expression plasmids (pWT511, pWT551 and pWT571) is described. The Tc-resistance gene is under the control of the Trp-operator. In pWT511, the HindIII site is located several codons after the ATG and allows production of a fusion protein with few N-terminal amino acids from the Trp leader peptide. The bom or Mob site is lost. Hosts: E.coli. Related vectors: pAT153, EcoTrpPO, EcoTrp, pBR327, pWT511, pWT571. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
GenBank
L09154
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence