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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pWH1266

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
8900
Notes
Contains the following restriction sites (approximate kb from the EcoRI site): BamHI--0.38; PvuII--2.1, 6.6; AccI--0.65, 4.5, 5.7, 6.7; HincII--0.65, 3.7, 8.4. The 2.2 AccI/HincII fragment is essential for replication in A. calcoaceticus. The cryptic plasmid pWH1277 isolated from A. calcoaceticus lwoffi was linearized with PvuII and cloned into the PvuII site of pBR322. Ampicillin and tetracycline resistance are both expressed in A. calcoaceticus. Restriction digests of the clone give the following sizes (kb): BamHI--8.8; PvuII--4.4 (doublet); AccI--3.8, 2.9, 1.2, 0.9; HincII--4.6, 3.1, 1.1; EcoRI--8.8. (ATCC staff) Shuttle vector. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208940 Hosts: E.coli HB101, Acinetobacter calcoaceticus, E.coli, Acinetobacter calcoaceticus BD4. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77092
GenBank
M36473
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified