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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pVT66

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
7000
Notes
Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid. (personal communication) Cloning into the HincII, PstI, PvuI, or ScaI sites inactivates ampR. Cloning into the SmaI site inactivates cmlR. The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR. Shuttle vector. Restriction digests of the clone give the following sizes (kb): PstI--7.0; PvuI--7.0; SmaI--7.0; BamHI--4.7, 2.4. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JM109, Haemophilus influenzae, E.coli, Haemophilus influenzae BC200. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77246
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified