Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pVT65
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6500
- Notes
- Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid. (personal communication) Cloning into the HincII, PstI, PvuI, ScaI, or SspI sites inactivates ampR. Cloning into the BalI or NcoI sites inactivates cmlR. The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR. Shuttle vector. Restriction digests of the clone give the following sizes (kb): PstI--6.6; PvuI--6.6; SspI--3.7, 2.9; PvuII--6.2, 0.46; BamHI--4.0, 2.4. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JM109, Haemophilus influenzae, E.coli, Haemophilus influenzae BC200. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77245
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified