Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pVEX1211
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3500
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; PstI--2.1, 1.1, 0.35. (ATCC staff) Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages. To be used with pVEX2211 or pVEX2212 (ATCC 77422, 77423). Differs from pVEX1212 (ATCC 77421) only in the orientation of the polylinker. The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when spectinomycin selection is imposed in a host not expressing repA. In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates [pVEX1211(pVEX1212) and pVEX2211(pVEX2212)] provide two loxP sites for site-specific recombination that deletes the intervening target sequence. The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir. [1] Growth: 3 plus streptomycin (20 mcg/ml) (ATCC medium number 1445) 37C Deposited by: Figurski D.H. Hosts: E.coli, E.coli BR2943. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77420
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified