Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pUXV3
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6000
- Notes
- Shuttle expression vector using the Ustilago maydis gap (glyceraldehyde phosphate dehydrogenase) promoter to drive expression of the cloned insert. One of a series of vectors (ATCC 77463-77465 and 77468-77471) differing only in the selectable marker and the orientation of the gap promoter. The order of the major features in the plasmid is: Ustilago maydis ARS - ampR - pMB1 ori - 5' lacZ fragment - EcoRI - SacI - gap promoter -> BamHI <- cbxR - 3' lacZ fragment. [2] The sequence conferring resistance to carboxin (cbxR) is a mutation of an iron-sulfur protein subunit of the Ustilago maydis succinate dehydrogenase gene. [3] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: J.A.Bruenn Hosts: Ustilago maydis, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77465
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified